| Venue: Centre Aquatique Sportif CIBIC |
| Final 10:36 | time | Bib | | 1. Hellen Andrea Montoya Rios |  COL | 43.370 | 27 | | 2. Erin Jackson |  USA | 43.714 | 36 | | 3. Ingrid Factos Henao |  ECU | 43.875 | 31 | | 4. Veronica Elias |  MEX | 43.940 | 34 |
|
|
| | | First 2 progressed to Final. |
| Heat 1 09:50 | time | Bib | | 1. Hellen Andrea Montoya Rios | COL | 43.506 | 27 | | 2. Erin Jackson | USA | 43.597 | 36 | | 3. Rocio Berbel Alt |  ARG | 43.666 | 22 | | 4. Dalia Soberanis Marenco |  GUA | 44.079 | 33 | | 5. Jennifer Monterrey |  CRC | 44.664 | 29 |
| Heat 2 09:52 | time | Bib | | 1. Ingrid Factos Henao | ECU | 42.695 | 31 | | 2. Veronica Elias | MEX | 42.740 | 34 | | 3. María José Moya |  CHI | 42.748 | 25 | | 4. Yarubi Bandres Garcia |  VEN | 42.877 | 38 | | 5. Valérie Maltais |  CAN | 43.420 | 24 |
|
|
|
|
