| Announced athlete | Qualified |
| World Championships 2014 - 4 |
| --. Jin Jong-oh | South Korea | Jin Jong-oh |
| --. Jitu Rai | India | Jitu Rai |
| --. Pang Wei | China | Pang Wei |
| --. Hoàng Xuân Vinh | Vietnam | Hoàng Xuân Vinh |
| American Continental Championships 2014 - 1 |
| --. Jorge Grau | Cuba | Jorge Grau |
| World Cup 3 2015 ISSF - 2 |
| --. Wang Zhiwei | China | Zhang Bowen |
| --. Han Seung-woo | South Korea | Park Dae-hun |
| World Cup 5 2015 ISSF - 2 |
| --. Damir Mikec | Serbia | Damir Mikec |
| --. Jay Shi | United States | James Henderson |
| World Cup 6 2015 ISSF - 2 |
| --. Giuseppe Giordano | Italy | Giuseppe Giordano |
| --. Tomoyuki Matsuda | Japan | Tomoyuki Matsuda |
| European Games 2015 - 1 |
| --. Pavol Kopp | Slovakia | Pavol Kopp |
| Pan American Games 2015 - 1 |
| --. Marko Carrillo | Peru | Marko Carrillo |
| European Championships 2015 - 2-1 |
| Exchanged | Italy | Francesco Bruno |
| Italy secured an additional quota place in the men's 50 m pistol at the 2015 European Shooting Championships, but the Italian Olympic Committee and the Italian Sport Shooting Federation (UITS) chose to exchange it with the men's 25 m rapid fire pistol instead based on performances throughout the qualifying period. The slot was occupied by Riccardo Mazzetti. |
| --. Denis Koulakov | Russia | Sergey Chervyakovskiy |
| World Cup 7 2015 ISSF - 2 |
| --. Pablo Carrera | Spain | Pablo Carrera |
| --. Prakash Nanjappa | India | Prakash Nanjappa |
| Oceania Championships 2015 - 1 |
| --. Daniel Repacholi | Australia | Daniel Repacholi |
| African Championships 2015 - 1 |
| --. Samy Abdel Razek | Egypt | Samy Abdel Razek |
| Asian Olympic Qualifying Tournament 2016 - 2 |
| --. Kim Song-guk | North Korea | Kim Song-guk |
| --. Kim Jong-su | North Korea | Kim Jong-su |
| Additional - 21 |
| _Exchange of quota places - 1 |
| --. Almeida Júlio | Brazil | |
| Host nation Brazil had been awarded a minimum of nine quota places in select events with only six shooters using the vacancies for the Games. Almeida Wu and Rippel attained a direct nomination to the Olympic team to leave the vacancies unused for reallocation. With a lack of shooters in women's 10 m air pistol, the Brazilian Shooting Federation decided to exchange it with the men's 50 m pistol, which was awarded to Júlio Almeida. |
| Tripartite Commission Invitation - 1 |
| --. Rudolf Knijnenburg | Bolivia | |
| Athletes qualified in other events - 19 |
| --. Felipe Almeida Wu | Brazil | |
| --. Samuil Donkov | Bulgaria | |
| --. Tsotne Machavariani | Georgia | |
| --. Miklós Tátrai | Hungary | |
| --. Vladimir Issachenko | Kazakhstan | |
| --. Rashid Yunusmetov | Kazakhstan | |
| --. Johnathan Wong | Malaysia | |
| --. Naung Ye Tun | Myanmar | |
| --. David Muñoz | Panama | |
| --. João Costa | Portugal | |
| --. Vladimir Gontcharov | Russia | |
| --. Atallah Al-Anazi | Saudi Arabia | |
| --. Dimitrije Grgić | Serbia | |
| --. Juraj Tužinský | Slovakia | |
| --. Yusuf Dikeç | Turkey | |
| --. Keleş İsmail | Turkey | |
| --. Oleh Omelchuk | Ukraine | |
| --. Will Brown | United States | |
| --. Trần Quốc Cường | Vietnam | |
| Total - 41 Shooters. |